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KMID : 0880220070450060583
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Journal of Microbiology
2007 Volume.45 No. 6 p.583 ~ p.589
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Effect of fur on pyrC Gene Expression
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Chai Sang-Ho
Song Chang-Kyu
Kim Seong-kwun
Park Jun-Ho
Wee Se-Chan
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Abstract
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The promoter region of pyrC (dihydroorotase) gene of Escherichia coli was shown to have Fur protein binding properties by gel retardation assay. In vivo regulation of the pyrC expression was studied by measuring dihydroorotase activity and beta-galactosidase level in the fur+ and fur- genetic background. The expression of chromosomal dihydroorotase activity and beta-galactosidase activity of pyrC-lacZ fusion plasmid was repressed to about 30% and 17%, respectively in the fur+ strain compared to those in the fur- strain. Divalent ions such as Fe2+ and Zn2+ were not required for the repression. PyrC expression was also reduced to one half by 1 mM uracil. The effect of uracil was independent on the fur gene.
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KEYWORD
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Fur, pyrC expression, iron, dihydroorotase
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